Objective: Evaluation of Anti-urolithiatic and Larvicidal activity of Alternanthera sessilis. Method: The whole plant of Alternanthera sessilis were extracted using ethanol as solvent. Then it was evaluated for its phytochemicals and later on in vitro anti-urolithiatic study was conducted on the plant using the methods titrimetry, simultaneous flow static model, turbidimetry and gravimetric. The plant showing larvicidal effect was determined by larvicidal assay method. Result: The ethanolic extract of the plant showed the presence of various phytochemicals like phenols, flavonoids, tannins, sterols, saponins. The anti urolithiatic activity conformed that the plant can effectively mineralise calcium oxalate in a dose dependent manner when compared to control and standard. The plant also possesses larvicidal activity and the percentage mortality exhibited a dose dependent manner. Conclusion: The ethanolic extract of the plant possessed anti- urolithiatic as well as larvicidal activity.

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