Background: Diabetes is a group of metabolic diseases characterized by high blood glucose level resulting from defects in insulin secretion, insulin action, or both. The chronic long term hyperglycemia of diabetes is associated with long-term damage, dysfunction, and failure of different organs, especially the eyes, kidneys, nerves, heart, and blood vessels. Aim and Objective: To assess the knowledge and self-care practice regarding diabetes among patient diagnosed with diabetes mellitus in selected hospitals Kamrup (M), Assam Methods and materials: A descriptive survey design was used to accomplish the objectives. 100 diabetic patients were selected as sample by using purposive sampling technique. Structured knowledge questionnaire and inventory checklist were used to assess the level of knowledge and self- care practice. Results: The study revealed that out of 100 sample, majority 84(84%) had moderate knowledge, and majority 52 (52%) had adequate practice regarding management of diabetes. There was moderate positive correlation between knowledge and practice score as the calculated r value is 0.43.There was significant association between the level of knowledge and practice with the demographic variables such as educational status, occupation, place of residence and duration of diabetes. There was also significant association between the level of practice with the demographic variables such as availability of glucometer a home, attended session of health education on diabetes and current treatment for diabetes. Conclusion: Hence, proper awareness regarding self-care management of diabetes is needed in order to improve the knowledge and self-care practice among diabetic patient. Key words: knowledge, self-care practice, Diabetes mellitus, Information booklet.