We report the case of a 42-y-old male with an isolated cervical lymphadenopathy due to Brucella melitensis. The diagnosis was established by isolation of B. melitensis in a lymphatic specimen obtained by fine-needle aspiration and confirmed by serological test results showing high levels of Brucella agglutinins. Mycobacteria-specific cultures were negative. Treatment with streptomycin and doxycycline resulted in complete healing. Exceptionally, and only if the epidemiologic context supports it, brucellosis should be considered in the differential diagnosis of lymphadenopathy.

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