Introduction: Diabetes is the most common endocrine disease, which affects about 150 million people worldwide. Disruption of the immune system and other factors reduces the resistance to microorganisms in diabetic patients. Therefore, the aim of this study was to determine the prevalence of Candida and its strains in diabetic and non-diabetic patients. Materials and methods: This cross-sectional and analytical study was conducted on 60 hospitalized patients, among whom 30 were diabetic and 30 were non-diabetic during June 2021 to December 2021. According to the location of the infection, sampling was done using a swab or a slow scalpel blade. Demographic information of patients such as: Age, gender, level of education, occupation, site of infection, underlying disease and drug use were collected. Results: The status of the frequency of isolated oral Candida showed no significant relationship between gender and the frequency of oral Candida (P=0.593). In our study there is no association between insulin intake, artificial teeth, smoking, antibiotic use, place of residence, level of education, age, duration of diabetes with Candidiasis (P>0.05). The results showed a significant relationship between the frequency of oral Candida isolated from diabetic and nondiabetic patients and the type of diabetes observed (P=0.008). Conclusion: In our research, there was a significant relationship between oral Candida infection and the type of diabetes and no significant relationship between diabetic and non-diabetic patients with the frequency of Candida and its strains.