The purpose of this study was to determine the qualitative and quantitative characteristics of female Bali cattle in Atinggola District, North Gorontalo Regency, Gorontalo Province. This study used a field experiment method in the form of direct observation and measurement of female Bali cattle based on the total population and livestock density in each village in Atinggola District. A total of 122 female Bali cattle aged 2-4 years were used as samples for observation and measurement. Bali cattle aged 2 3 years have a white pattern on the butt (butt mirror) with clear boundaries of 74.64% and 25.33% without clear boundaries. The white pattern on the feet (socks) is clearly visible with the skin color on the legs 73.33% and 26.67% there is no dividing line between the body color and the white pattern on the legs (socks). Bali cattle aged 3 4 years have a white pattern on the butt (butt mirror) which is clearly demarcated by 63.83% and 36.17% without clear boundaries. The white pattern on the feet (socks) is well defined with body hair color 68.09% and 31.91% there is no clear boundary between body color and pattern color. Female Bali cattle aged 2 3 aged 5.81-25.15, the average body length, chest circumference, and height were 93.91±9.29, 148.78±20.40 and 106, respectively, 10±6.16. Bali cattle aged 34 have a diversity of body size of 5.29-35.17%. The average values of chest circumference, body length, and height were 98.73±10.31, 144.22±14.62, 144.22±14.62, respectively. Female Bali cattle from Atinggola District, North Gorontalo Regency, Gorontalo have qualitative characteristics that do not deviate from native Indonesian Bali cattle. Body size diversity of female Bali cattle aged 2 3 was 5.81-25.15, while Bali cattle aged 3 4 had body size diversity of 5.29-35.17%

Bats have been known as natural reservoirs for potential emerging infectious viruses, such as Lyssaviruses, Coronaviruses, Ebola viruses, Nipah virus, and many others. Because of their abudance in population, wide distribution and mobility, bats have a greater risk as source for zoonotic transmission than other animals. Despite the facts of their role as reservoirs for many pathogens, not until an epidemic of Severe Acute Respiratory Coronavirus (SARS-CoV) in 2003 and Middle-East Respiratory Syndrome Coronavirus (MERS-CoV) in 2012, that people pay much attention about coronavirus in bats. SARS-like virus also found in bats with a higher prevalence rate. This study aims to detect the coronavirus of bats in Gorontalo province Indonesia, characterization at the molecular level of the coronavirus genome and determining the level of kinship (through trees filogenetic). This study was conducted as part of bigger PREDICT Indonesia project, in particular to examine coronavirus in bats from Gorontalo province, Indonesia.  As many as  95 rectal swab samples collected from flying foxes (Pteropus alecto) were analyzed in the laboratory using Consensus Polymerase Chain Reaction (PCR) technique to amplify the target sequence from RNA-dependent RNA Polymerase (RdRp) gene with 434 basepair product, resulted 24 samples determined as presumptive positive. Eight out of 24 presumptive positive samples by PCR were analyzed further by nucleotide sequencing and confirmed coronavirus positive. Phylogenetic tree analyses to the eight coronavirus confirmed-sequences were constructed with MEGA-6.0 . The conclusion was 24 out of 95 samples suggested as presumptive positive to Bat CoV. Eight out of 24 samples were analyzed further by nucleotide sequencing and have similarities in the kinship. Three samples had the 98% nucleotide identity to BatCoV from Indonesia and five samples were 85-88% nucleotide identity to BatCoV from Thailand.

The research objective was to determine the phenotypic diversity of the qualitative characteristics of Kampung chickens. This research uses a descriptive statistic method. A total of 300 adult (unsex) chickens aged 6 months were used for this study. Qualitative characteristics include: color feather, color pattern, comb shape, fliker, and color variations. The results showed that the gene frequencies of colored feathers and colorless feathers from Kampung chickens were 0.96% and 0,04. The gene frequencies for the color feather pattern were 0.61, 0.43, and 0,03 for the columbian, wild and black color patterns. The gene frequencies for striated and plain hair were: 0.29 and 0.71. The gene frequencies for silver and gold feathers are 0.30, and 0.71%. The Gene Frequencies for Single Comb and Pae are 0.60 and 0.40. The phenotypic frequency of the color pattern of the Kampung chickens was 43.33%, 38.00%, and 12.00%, for wild, Columbia and black pattern. Phenotypic frequency of lurik and plan were: 81.34% and 18.66%. The phenotypic frequency of silver and gold feather were 31,89%, and 68.11%.  The phenotypic frequencies of the comb shape single, pea, rose, and walnuts were 36%, 27,82%, 18,51% dan 17,67%. The population of Paguyaman chickens has a phenotype of colored feathers, the color feather pattern are wild, single comb, yellow shank color, and these characteristics are dominant compared to other characteristics.

This research is the first step, with aims to determine the production and reproduction ability of Crossbreding between male native chicken with female Isa brown strain leghorn, to be the parent in the next stage of research . This study used  3 male native chicken 12 -24 months of age. and  and 28 females of Leghorn chicken 14 month of ege,  with body weight 1100-1200 gram, crossbreding using artificial Insemination methode, semen domestic chicken dilution rate using physiological NaCl,  ratio sperm dilution 1 : 4. They were put in grower flocks with density of one birds per cage. Feeds during experiment were given in phases, starter Feeds  (Protein 21% and Energy 3000 kcal/kg), for layer (Protein 17% and Energy 2900 kcal/kg). Drinking water during experiment were given adlibitum. The result of the study illustrate crossbreeding between male native chicken and female leghorn chiken Isa Brown strain is  egg weight: 51.50-54.17 gram, egg index: 0.76 to 0.79%., Hatching weight: 39.34-39,39 grams, egg fertility 65,70-78,00%, hatchability 76.83-78.75%. In conclusion: egg weight of 52.42 eggs, egg index 0.78, eggs fertility 74.43%. hatchability 77,93%, and F1 body weight of 1 days of ege :  39.37 gram, and heritability DOC weight 0.43

The aim was to determine the heritability of egg weight, hatch weight, age of a week body weight in crossbred chickens. This research was conducted from August-November 2017, in Tumbihe Village, Kabila District, Bone Bolango District, Gorontalo province. The research material was eggs and DOC originating from a cross between male Kampung chicken and female Leghorn chicken, Isa Brown strain, these eggs were weighed and hatched in a hatchery machine. The parameters measured were egg weight heritability, hatch weight, 1 week body weight in crossbred chickens. Data were analyzed by descriptive statistics. The results of this study are the heritability of egg weight in the population is 0.533 ± 0.03 and the variance coefficient is 6.16%. The heritability of hatch weight in the population is 0.532 ± 0.02 and the variance coefficient is 4.33%. While the heritability value of 1 week age weight in the population is 0.530 ± 0.02 and the variance coefficient is 3.26%.