The 26 December 2004 earthquake and tsunami resulted in over 100,000 damaged or destroyed homes and over 500,000 internally displaced people in northern Sumatra. Reconstruction and recovery from these massive losses requires the coordination of many stakeholders, including multiple levels of government, nongovernment relief organizations, donors, and the people of northern Sumatra. Although efforts have been taken by the Government of Indonesia to develop standards for the reconstruction of houses and establish a coordinating body, the reconstruction effort in Sumatra still faces many challenges. A broad range of housing types, with varying degrees of construction quality, have been constructed as part of the recovery effort. A field study team visited Banda Aceh, Meulaboh, and Nias seven months after the December event and documented the process of reconstruction, the interaction of the stakeholders, and the types of housing construction.
Background
Colorectal cancer (CRC) is still considered one of the prevalent cancers worldwide. Investigation of potential biomarkers for early detection of CRC is essential for the effective management of patients using therapeutic strategies. Considering that, this study was aimed to examine the changes in lncRNA FOXD2-AS1 expression through colorectal tumorigenesis.
Material and Methods
Fifty CRC tumor tissues and fifty adjacent normal tissue samples were prepared and involved in the current study. Total RNA was extracted from the samples and then reverse transcribed to complementary DNA. Next, the expression levels of lncRNA FOXD2-AS1 were evaluated using real-time PCR in CRC samples compared to normal ones. Also, receiver operating characteristic curve (ROC) analysis was used to evaluate the diagnostic value of FOXD2-AS1 for CRC.
Results
The obtained results showed that the expression level of FOXD2-AS1 gene was significantly (p < 0.0001) up-regulated in tumor tissues compared to normal marginal tissues. Also, a significant correlation was observed between higher the expression of FOXD2-AS1and the differentiation of tumor cells. Furthermore, ROC curve analysis estimated an AUC value of 0.59 for FOXD2-AS1, suggesting its potential as a diagnostic target.
Conclusion
Taken together, the current study implied that tissue-specific upregulation of lncRNA FOXD2-AS1 might be appropriate diagnostic biomarkers for CRC. Nonetheless, m studies are needed to validate these results and further illustrate FOXD2-AS1 function through colorectal tumorigenesis.
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