Evaluation of the level of anti-sperm antibodies, some cytokines and zinc for oligozoospermia patients, asthenozoospermia patients and normozoospermia patients. Semen samples were collected from asthenozoospermic, oligozoospermic, and normozoospermic men who attended the fertility center. The collected samples of normozoospermia patients were (15), Asthenozoospermia patients were (15), and Oligozoospermia patients were (13). Seminal plasma for all study groups was evaluated for interleukin-1, interleukin-6, and zinc levels. The result showed there were statistically significant differences (p < 0.05) in sperm concentration, progressive motility, and liquefaction time in the asthenospermia and oligospermia groups compared with the normospermia group. This study found that antisperm antibody, interleukin 1, and interleukin 6 were higher in the oligozoospermia and asthenozoospermia groups than in the normozoospermia group. The levels of antisperm antibodies and interleukins increase, while zinc levels decrease in the seminal plasma of patients with oligospermia, as well as those with asthenospermia, compared to patients with normospermia.

Studying the efficiency of the swim up from pellet technique in reducing antisperm antibody and some cytokine levels in seminal plasma. Semen samples were collected from oligozoospermic and Normozoospermic who attended a fertility center. The collected samples of normozoospermia patients were (15 (and oligospermia patients were (13). antisperm antibody, interlukine-1, interlukine-6, and zinc levels were evaluated in seminal plasma before and after semen processing for all study groups. The results revealed that there was a statistically significant decrease (p < 0.05) in antisperm antibody, interleukin 1Β (IL-β), interleukin (IL-6), and zinc. After sperm preparation by swim up from pellet compared with before sperm preparation, The result illustrates there was a statistically significant decrease (p < 0.05) in sperm concentration and leucocytes count while there was significant increase (p>0.0.5) in progressive motility, normal morphology after preparation by swim up from pellet compared with before the preparation. In concluded Processing by swim-up techniques decreases sperm concentration, and also shows an increase in sperm normal morphology and progressive motility in normospermia, oligospermia, and asthenospermia groups. Antisperm antibody, interleukin 1 (IL-), interleukin 6 (IL-), and zinc levels are all reduced.