This study included the synthesis of four compounds of maleimide derivatives. Novel compounds (K1-K4) resulted from the reaction between N-substituted maleimide and aryl hydrazide (benzohydrazide, p-toluic hydrazide or isonazide). The novel maleimide derivatives were identified using infrared spectroscopy (FT-IR), 1H- and 13 C-nuclear magnetic resonance (NMR) spectroscopy, and mass spectrometry, as well as the melting point of the preparing compounds. The MTT assay were used to examine the anti-breast cancer (MCF-7) activities of four compounds. The compounds K2 and K4 demonstrated anti-cancer activity against breast cancer cells.