No abstract

Antioxidant activity of several classes of lichen metabolites were assessed in the in vitro superoxide radical (SOR), nitric oxide radical and 2,2-diphenyl-1-picrylhydrazil radical scavenging assays. The despsides sekikaic acid and lecanoric acid showed promising antioxidant activity in SOR assay with IC₅₀ values of 82.0 ± 0.3 µmol and 91.5 ± 2.1 µmol, respectively, while the depsidone lobaric acid exhibited an IC₅₀ value of 97.9 ± 1.6 µmol, all relative to the standard, propyl gallate (IC₅₀ = 106.0 ± 1.7 µmol). One of the most abundant mononuclear phenolic compounds, methyl-β-orcinol carboxylate was found to be a potent NO scavenger (IC₅₀ = 84.7 ± 0.1 µmol), compared to the standard rutin (IC₅₀ = 86.8 ± 1.9 µmol).

BackgroundThe medicinal plants Vincetoxicum arnottianum (VSM), Berberis orthobotrys (BORM), Onosma hispida (OHRM and OHAM) and Caccinia macranthera (CMM) are used traditionally in Pakistan and around the world for the treatment of various diseases including cancer, dermal infections, uterine tumor, wounds etc. The present study focuses on the investigation of the selected Pakistani plants for their potential as anticancer agents on human bone and breast cancer cell lines in comparison with non-tumorigenic control cells.MethodsThe antitumor evaluation was carried out on human bone (MG-63, Saos-2) and breast cancer cell lines (MCF-7, BT-20) in contrast to non-tumorigenic control cells (POB, MCF-12A) via cell viability measurements, cell cycle analysis, Annexin V/PI staining, microscopy based methods as well as migration/invasion determination, metabolic live cell monitoring and western blotting.ResultsAfter the first initial screening of the plant extracts, two extracts (BORM, VSM) revealed the highest potential with regard to its antitumor activity. Both extracts caused a significant reduction of cell viability in the breast and bone cancer cells in a concentration dependent manner. The effect of VSM is achieved primarily by inducing a G2/M arrest in the cell cycle and the stabilization of the actin stress fibers leading to reduced cell motility. By contrast BORM’s cytotoxic properties were caused through the lysosomal-mediated cell death pathway indicated by an upregulation of Bcl-2 expression.ConclusionsThe antitumor evaluation of certain medicinal plants presented in this study identified the methanolic root extract of Berberis orthobotrys and the methanolic extract of Vincetoxicum arnottianum as promising sources for exhibiting the antitumor activity. Therefore, the indigenous use of the herbal remedies for the treatment of cancer and cancer-related diseases has a scientific basis. Moreover, the present study provides a base for phytochemical investigation of the plant extracts.Electronic supplementary materialThe online version of this article (doi:10.1186/s12906-016-1215-9) contains supplementary material, which is available to authorized users.