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In a prospective study, 132 patients were investigated for yeast infection of burn wounds. Ten patients (7.6%) were infected with Candida species. All patients with yeast infections were also infected with bacteria with the exception of one patient who was infected with Candida tropicalis alone. The predominant yeast recovered was Candida krusei. Yeast infection was found to be more common in the younger age group. The isolation of a Candida species alone from one patient and Candida isolation from patients with sepsis in burn wounds indicate a significant role for yeasts in the production of infection in burn wounds. Therefore, special cultures for yeasts are recommended for all cases of burn wound infection.

Twenty five sputum and throat swabs were collected from 25 asthma and bronchial allergic patients from Allergy and asthma Centre Governorate of Basra/Iraq beside 25 indoor air samples from their houses. Another 25 samples were collected from normal individual and from their indoor air. These Samples were cultured on Sabouraud dextrose agar at 25 and 37C o ,seventeen genera were identified in patient samples and (23) genera in their indoor air samples while 4and5 species were identified from normal individual and from their indoor air samples respectively beside yeast colonies and sterile mycelium were recorded in all samples. Hyphomycetes are the dominant group in all tested samples, it was 67.5% in patient samples, and %75.38 in their indoor air samples beside 66.66% and 81.25% in normal individual and in their indoor air samples respectively. The prominence value of yeasts was 735.66 in patient samples and 69.86 in their indoor air samples while it was 546.62 and 405.69 in the normal individuals and in their indoor air samples respectively. According to the filamentous isolates, the genera Aspergillus, Penicillium, Alternaria, Ulocladium, Paecilomyces, and Cladosporium have the highest prominence value in the patients samples and in their indoor air samples. In normal individuals, Aspergillus and sterile mycelium showed the highest prominence value and Penicillium, Aspergillus, Cladosporium and Ulocladium in their indoor air samples.

Introduction: A great progressing occurs in microbiology Ulocladium sp. after culture media have been used in culturing, isolation, identification, and reservation of microorganisms. According to their components, culture media were divided into natural, synthetic, and semisynthetic, the natural medium contain almost all the nutritional requirements but in non-defined quantities. In the current work, the validity of Typha domingensis pollens as a new substrate for fungal culture medium was tested. Several physical and chemical characteristics of medium were detected. The growth of nine fungal isolates were evaluated on semisolid and broth media. Materials and Methods: Ten gram of starchy powder free from spike tissue + 15gm agar were used for one liter of Typha pollens agar (TPA). The growth of local isolates of Candida sp., Rhodotorula sp., Aspergillus niger, , Cladosporium sp., Trichoderma sp., Penicillium sp., Chrysosporium sp., and Microsporum sp. were tested on (TPA), and (PCA). Biomass of filamentous isolates was estimated by TP broth and PC broth.ANOVA test at level 0.05 was followed to clarify the significant increasing of biomass. To qualify the nutritional value of Typha pollens, the percentage oftotal nitrogen , carbon , phosphorus , and potassium were estimated . pH of TPA were detected as well as its color, transparency , and gelatin texture were practically evaluated . Results and Discussion : The chemical composition analysis showed that pollens powder contain C=58%, N=2.16%, P= 0.19%and K=3.36%. The pH was 6.72 at room temperature . Typha pollens agar support growth of all tested fungal isolates. The semisolid TPA had a typical characteristic for culturing and diagnosis of fungi including a suitable gelatin texture and a pale transparent yellow color . All tested fungi except Penicilliumsp. showed higher growth on TPA , on the other side the fungal biomass increased significantly by TP broth in comparison with PC broth (ANOVA test at 0.05 level). Conclusions : Pollens of cattail (T. domingensis ) provide a suitable environment , and nutritive requirements for fungi .It can be usedeasily and successfully as essential substrate for preparing culture medium for primary isolation of fungi. A farther studies were needed to gain a view about using T.domingensis pollens to isolate pathogenic fungi and bacteria.

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