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This study was carried out on 26/5/2019 until 17/6/2019 At local hatchery (College of Agriculture / University of Karbala), to investigate of Different spraying protocols on the eggs of local chicken with the eggs of local chicken with different solutions at variant periods on embryonic mortality during incubation period and on the hatchability of setting eggs. 600 eggs (55 g weight ) used in this study from local chicken divided into three groups each group sub divided into 5 groups spraying by distilled water, olive oil, ethanol alcohol 70%, egg albumen and not treated groups that represented as GW, GO,GE,GC. first group G1 (n = 120) was sprayed at the first day of incubation period, the second group G2 was spraying at beginning of the 2nd week of incubation at aged 7 days, the third group G3 in which the eggs was sprayed at the beginning of 3rd week of incubation at aged 14 days. The results recorded significant reduction of early embryonic mortality in G1 and GC The statistical analysis of the in all groups spraying by ethanol alcohol GE, water group GW and control groups GC. At 14 days of the incubation period, there were no significant differences. At 15-21 days of incubation period, late Embryonic mortality was not recorded in groups treated olive oil, water and albumen when compared with groups treated with alcohol or control. Conclusion, that spraying Iraqi local eggs with water and olive oil at 14 days of incubation period caused high percentages of hatchability of setting and fertilized eggs recorded 100% compared with different materials that used in current research.

The current experiment was aimed to explore the influencing of the sex-linked dwarf gene on some fertility hormones and biochemical profile in normal local and dwarf roosters. Thirty normal local and dwarf roosters at age 28 weeks were housed at opened system for 6 weeks. At end of 6th week, blood samples collected from all roosters and centrifuged to obtained sera for measuring the concentrations of Interstitial cell stimulating hormone ‘ICSH’, Follicle stimulating hormone ‘FSH’ and testosterone hormone, total protein, albumen, globulin, glutamic oxaloacetic transaminase ‘GOT’, Alkaline phosphatase ‘ALP’, and glutamic pyruvic transaminase ‘GPT’. The statistical analysis documented significant (P<0.05) decline in FSH and testosterone hormone in dwarf group roosters compared with normal local roosters. As well as, the level of total protein and globulin registered significant decline (P<0.05) in dwarf group roosters in comparison with normal local group roosters. On the other hand, the level of hepatic enzymes, GOT, GPT and ALK, recorded a significant (P<0.05) elevation in dwarf group roosters when compared with normal local group roosters. Concluded, that the sex-linked dwarf gene could be affected on hormonal fecundity profile and some biochemical traits in dwarf roosters.

The current experiment was aimed to explore the effect of electrical stimulation (ES) on glucose concentrations, cholesterol levels and triglyceride levels in blood and its effect on wound healing in intact and induced diabetes rabbits. 40th male rabbit divided to five treatment, T1 intact animal as control (positive control), T2 intact animal treated with (ES), T3 diabetic rabbits treated with (ES), T4 diabetic rabbit treated with insulin and T5 diabetic rabbits with no treatment as a negative control. Three sub groups were designed as well, T2*, T3*, T4* each one of them is the same main group but with artificial wound on some of its animals. the results were shown a significant decreasing p<0.05 in blood glucose concentration in T3 in comparison with T4 and T5. The cholesterol levels increased significantly p<0.05 in T4 in compared with T3. The concentrations of triglycerides recorded significant p<0.05 decreasing T3, T4 and T5 in compared with T1 and T2. In another hand the ES had a very obvious effect on wound healing in both T2* (intact animals) and T3* (diabetes animals) in comparison with T4* (insulin treated diabetes animals).

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