To determine the concentrations of ochratoxin A in wheat, and its derivatives, 81 samples, including durum wheat (n ¼ 27), common wheat (n ¼ 12), semolina (n ¼ 29) and flour (n ¼ 13) were collected from regions of Algeria. The results showed that 62 of the 81 samples (76.54%), were contaminated with quantities of ochratoxin A ranging from 0.84 to 34.75 mg/kg. The results showed that 69.23% of wheat grains were contaminated with ochratoxin A (0.21-27.31 mg/kg). Ochratoxin A were detected both in semolina and flour manufacturing process (0.16-34.75 mg/kg). Ochratoxin A level seem to be a serious public health problem in Algeria.