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S RB cytotoxicity assay was performed for different extracts of Hamelia patens Jacq. family Rubiaceae; crude flowers (CF), crude leaves (CL), chloroform (Chl.L) and methanol (Me.L) fractions from leaves against liver (HepG-2) and breast (MCF-7) human carcinoma cell lines. Results demonstrated potent cytotoxic action against HEPG-2 for CF, Chl.L and Me.L with IC 50 = 47, 30 and 44.4 µg/mL, respectively. On the other hand, CF, CL, Chl.L and Me.L had exerted powerful effect against MCF-7 with IC 50 = 23.8, 25.5, 17.7 and 64 µg/mL, respectively. Chromatographic investigation of Me.L , resulted in the isolation and spectroscopic identification of rutin (1), isoquercetin (2) and soyasaponin Bb (3), which was isolated for the first time from the plant leaves and was identified by UPLC/ITMS/MS analysis. Comparative study for using ordinary method versus green methods of extraction was also inspected.

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