Objectives: The aim of presented study was evaluated the role of some immunological parameter associated with Graves' disease and comparison that with other non-autoimmune hyperthyroidism, by the following objectives: (1) measurement T3, T4 and TSH. (2) Evaluation of Anti-TSHR-antibody. (3) Estimation Anti-TPO antibody. Methodology: A case control study included patients with early diagnosis with thyrotoxicosis. Whole blood samples were collected of 73 cases in hormonal unite at Al-Sadder Medical City, Najaf, Iraq, in the period between May, 2011 to Oct., 2011. Out of the 73 hyperthyroidism patients, there were 48 females and 25 males, the patients' age range was between (15-50) years. All patients with hyperthyroidism under treatment and pregnant women excluded of the study. Statistical analysis was done by using SPSS version 20 in which include the followings: (1) ANOVA (analysis of variance) and LSD test (F-test). (2) persons correlation (r), coefficient for measurement data. Results: According to the results of this study, the female: male ratio (2.17:1) in GD patients and (1.37:1) with non-autoimmune hyperthyroidism group, which detects more frequent in females (68.5% and 57.9%, respectively) than males (31.5% and 42.1%, respectively). The age group between (36-50) years with GD it is the most vulnerable age group was recorded (66.7%), followed (26-35) years age group (20.4%) and (13.0%) in age group (15-25) years. However, hormonal tests (T3, T4 and TSH) levels were highly significant difference (P<0.001) between GD patients and other groups (non-autoimmune hyperthyroidism and healthy control). Furthermore, the levels of Anti-TSHR antibodies were highly significant between GD patients (P<0.001), and both non-autoimmune hyperthyroidism patient and healthy control, while there was no significant difference between non-autoimmune hyperthyroidism and healthy control groups (P>0.05). Additionally, the serum levels of thyroid peroxidase (TPO) was a highly significant difference between GD patients and (non-autoimmune hyperthyroidism and health control) (P<0.01), while no significant difference between non-autoimmune hyperthyroidism and healthy control P>0.05). Anti-TSHR antibodies have a highly significant positive correlation (P<0.01) with (T3 and T4) hormones (0.583 and 0.536, respectively) and a highly significant (P<0.01) but negative correlation with TSH hormone (-0.475). Conclusion: Anti-TSHR antibodies assay is helpful in diagnosis of autoimmune GD, as they can be detected very early in AITD. They may predict the eventual development into AITD when founded in making them a good diagnostic marker. Recommendation: For adults, the best recommendation may be to put thyroid function testing (TSH, T4, T3) on your list of health care provider.

Objective: The study was designed to evaluation of effect Interleukin-6 and Tumor necrosis factor in seminal plasma to semen quality in infertile men with varicocele. Methods: A descriptive correlation study was conducted in specialized infertility center / teaching AL-Sadder medical city in Najaf city from first of April 2014 to the first of June 2014.Sample was selected that consisted of (75) infertile men with varicocele, and (25) healthy subject as control, their age between (20-40) years old. A questionnaire format and observational checklist were used which including marriage period, excluding from chronic disease like diabetic, cancer, sexual transmitted disease. The data were collected through the utilization of questionnaire and the structure interview technique with varicocele patients. Data was analyzed by using T-test and Z-test Results: The result of the study sample have revealed that 42% from all subjects has elevated Interleukin-6 concentration in seminal plasma ,and also the elevation Interleukin-6 level in seminal plasma has inversely correlation R = -0.58 with decreased in sperm motility ,also has inversely correlation R = -0.30 with low sperm count. From other hand 41% from all subjects has elevated Tumor necrosis factor levels in seminal plasma ,and also the elevation Tumor necrosis factor concentration in seminal plasma has inversely correlation R = -0.17 with decreased in sperm motility ,also has inversely correlation R = -0.25 with low sperm count. Conclusions: They study concluded there was a significant difference between elevation of Interleukin-6 and Tumor necrosis factor levels in seminal plasma in infertile men with varicocele, and they study concluded there was a significant correlation between increased levels of Interleukin-6 and Tumor necrosis factor in seminal plasma with reduce sperm count and motility . Recommendations: the study recommended that: Varicocele examination and diagnosis should be done to all infertile male. Varicocele patients with abnormal seminal fluid examination should be estimated concentration of Interluekin-6 and Tumor necrosis factor in seminal plasma

, 10 −5 of virus stock (8.860 log 10 RNA copies/µl). The virus cytopathic effect (CPE) was studied at 24, 48, 72, and 96 hr. Oncolytic effects of the virus were assessed by cytotoxicity assay, apoptosis analysis, and proliferation marker Ki-67. The growth inhibition percentage was determined by Enzyme-Linked Immunosorbent Assay (ELISA) reader using crystal violet staining assay and compared with the viral load measured by quantitative real time-PCR. Results The results of the study showed that measles virus had an inhibitory effect on the growth of human lung cancer cell line compared with untreated control group. There was highly significant positive correlation between viral load and PGI at 24, 48, 72, 96 hr (P < 0.001). The growth inhibition rate of a cell was associated with a decrement in the expression of the Ki-67 protein and increase in the ratio of early and late apoptotic cells in comparison with untreated control group. Conclusion Live attenuated measles virus strain induced cytotoxic effect against human lung cancer cell line (A549) by induction of apoptosis as an important mechanism of anti-tumor activity, in addition, it indicates a correlation between the quantity of MV genomes and percentage of growth inhibition. This relation has proved that measles virus had anticancer effect.

Epstein-Barr virus (EBV) is the causative agent of infectious mononucleosis and is implicated in the development of several human malignancies, such as nasopharyngeal carcinoma (NPC) and Hodgkin's Lymphoma (HL). While the incidence of Hodgkin's Lymphoma in Iraq is not yet investigated, according to the WHO Hodgkin's Lymphoma is one of the 10 most frequent types of cancer in Iraq (http://www.who.int/disasters/repo/10062.pdf). The present study aimed to determine the association between HL and EBV in middle Euphrates region of Iraq, and to determine whether it is related to certain age group or specific histologic subtypes. It also aims to compare the pattern with other developing or developed countries. This retrospective study includes 86 formalin-fixed paraffin-embedded tissue blocks from HL patients, these were compared with 35 patients with Reactive Follicular Hyperplasia, that were age and gender matched. All the samples belonged to the period between Jan.2008 to April2010. The presence of EBV DNA was investigated by polymerase chain reaction (PCR), while the expression of the oncogenic protein (LMP-1) in Reed-Sternberg cells by immunohistochemistry (IHC). The mean age group of HL patients was 28.5 old years with a median of 25 years old. There were 49 males and 37 females. Age group of 16-45 years was the largest one and represented 66.2%. The third decade took the peak incidence (26/86, 30.2%). patients aged less than 16 years were 17.5%, while 16.3% of patients were more than 45 years old. Mixed cellularity HL was the most common subtype represented 47.6% of all the cases, followed by nodular sclerosis (33.7%) while the lymphocyte-rich, and lymphocyte depleted subtypes were (8.1%, 7%) respectively, whereas Lymphocyte Predominant was seen in 3.48% of cases. EBV DNA was detected in 39.5% of the cases, while the expression of LMP-1 in Reed Sternberg cells was found in 85.3% of the EBV-positive HL. The highest rate of EBV was seen in the children age group (less than 16 years; 53.3%), while the lowest rate was among young adults (16-45 years; 35.08%). Older age group (more than 45 years) has a rate of EBV presented (39.5%) which was higher than the young adult patients, but lower than the children age group. Histologically, the most frequent viral association was observed in mixed cellularity subtype (56.1%), followed by lymphocyte-rich and lymphocyte depleted subtypes (42.9%, 33.3%) respectively. The least frequency of association was in nodular sclerosis type (20.7%), while lymphocyte predominant subtype showed negative relationship with EBV-positive HL. EBV positive cases were mostly males (40.81%) compared to females (37.8%). Citation Format: Dhiya S. Al-Shumary, Karrar A.A. Ali Beg, Alae A. Yaseen, Salman A. Al-Jibouri, Kaswer M. Al-Toriahi. Detection of EBV nucleic acid and expression of oncogenic protein LMP-1 in Reed-Sternberg cells of Hodgkin's lymphoma. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 4782. doi:10.1158/1538-7445.AM2013-4782 Note: This abstract was not presented at the AACR Annual Meeting 2013 because the presenter was unable to attend.