The VDBP amino acid sequence is divided into three domains and has 458 amino acids. Exon 11 in domain III has two SNPs, rs7041 and rs4588, each corresponding to one of the major VDBP kinds (VDBP1S, VDBP1F, and VDBP2). Transport of vitamin D3 to the liver and 25(O.H.)D to the kidney, 1,25(O.H.)2D transport to target organs is mediated by vitamin D-binding protein, which interacts with a particular vitamin D receptor (VDR) to explain biological activities. This study aims to determine whether the VDBP gene has three polymorphisms (rs17467825, rs7041, and rs4588) associated with women with osteoporosis in Mosul city. This study included (96) women, the age range between (35-55) years.The samples were divided into two groups, the first group included (74) women with osteoporosis, and the second group was considered a control group. In this study, DNA was extracted from the blood of all the samples included in the study (95) samples, using the method modified by the researchers. And detection of three different SNP of VDBP, (rs17467825) Polymorphism by ARMS-PCR, and (rs7041T) Polymorphism with (rs4588C) Polymorphism by RFLP-PCR. This study showed a connection between deficiency of vitamin D in women and mutation of the VDBP gene in location rs17467825.