Triply coupled high performance liquid chromatography using diode array detection and positive ion electrospray mass spectrometry of 2- and 3-hydroxypyridine is presented. Considerations of the physical method for coupling the two detectors, the influence of pH on retention times, the cone voltage of the mass spectrometer and the linear concentration ranges are described. Data from both detectors are aligned and interpolated. The analyte mass spectra are reduced to 20 significant masses. Principal components plots on the raw, normalised and standardised data, derivatives to determine composition 1 regions, deconvolution and procrustes analysis to compare data from both detectors are discussed. Common trends in both mass spectral and diode array chromatograms are interpreted. This paper represents a new approach to common processing of chromatographic data from two detectors.

No abstract

No abstract

No abstract

A new approach to deconvolution of diode array HPLC data is described. It is illustrated by the chromatograms of 2-and 3-hydroxypyridine undertaken at pH 4.8, 5.1 and 5.2 representing three different qualities of resolution from almost totally overlapping to well resolved. In all cases peaks are tailing, typical of many real situations. The first step is to select wavelengths using the normalised spectra at each point in time. Next a purity curve is obtained from these wavelengths, which is related to a real purity curve used for deconvolution. It is shown that the method is robust over the full resolvability range.