This study was conducted to investigate the effect of manganese toxicity by manganese chloride (MnCl 2) on the experimental animals and to evaluate the efficacy of phytic acid and/or ascorbic acid in attenuating the deleterious effect induced by manganese toxicity. For this purpose, thirty healthy rabbits weighing 1655±367.07g were divided into five groups each of six rabbits. Group 1; rabbits fed on commercial diet and normal water served as normal control; group 2; rabbits fed on commercial diet and received 200 mg/L of MnCl 2 in drinking water. Group 3; rabbits received MnCl 2 in drinking water (200mg/L) and fed on commercial diet supplemented with phytic acid (20 g /kg diet). Group 4; rabbits fed on commercial diet and received 200 mg/L MnCl 2 in drinking water and orally administrated with ascorbic acid (30mg/kg body weight daily). Group 5; rabbits received MnCl 2 in drinking water (200mg/L) and fed phytic acid (20 g /kg diet) and orally administrated ascorbic acid (30mg/kg body weight daily) by intragastric tube. Results showed that MnCl 2 intoxication significantly reduced haemoglobin (Hb) concentration and serum iron with a significant increase in total iron binding capacity. Also, it induced a significant increase in malondialdehyde (MDA) level accompanied by a significant decrease in reduced glutathione (GSH) concentration and superoxide dismutase (SOD) activity. Moreover, MnCl 2 intoxication caused a significant increase in serum alanine transaminase (ALT), aspartate transaminase (AST) activities. Also, serum urea and creatinine significantly elevated in MnCl 2-intoxicated group. An improvement was noticed in these altered parameters after oral administration of phytic acid and/or ascorbic acid.