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-The parameters of the modified mixed Ant Colony Optimization (ACO) -k-means image segmentation algorithm are considered. There have been investigated such parameters as n -the number of ants; heuristic coefficients of ACO algorithm and their dependence on the image scale and number of iterations before and after parameters correction. The proposed algorithm and sub-system for the study of coefficients, as part of the medical image segmentation system, have been implemented. Operation of the algorithm with and without the use of optimal parameters was applied. Optimal parameters were studied for 6 groups of MRI images: brain, heart, lungs, liver, bone structures, and others. The results are displayed in the final table. Images from Ossirix image dataset and real patients' images were used for testing.

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