The present study provides an evidence for the successful utilization of interspecies interactions to enhance antibiotic production in solid state fermentation that previously established in liquid cultures. Ground corn, wheat bran, rice husk and soya bean ground were examined in order to choose the substrate that support the prodigiosin production from Serratia marcescens. Results revealed that maximum production of prodigiosin was obtained in wheat bran medium, starting from early hours of incubation and reaching its maximum of product yield 47.5 mg.gds-1 (mg per gram of dry substrate) after 48 h of incubation. An enhancement by 2.3-fold in prodigiosin production was obtained as a result of introducing, separately, live and dead prokaryotic and eukaryotic microbial cells as elicitors to S. marcescens medium. The highest prodigiosin production (240mg.gds-1) was obtained when S. marcescens was cultured in wheat bran medium supplemented with sunflower oil (0.5ml.g-1 substrate) and live cells of Bacillus subtilis (0.4ml.g-1 substrate) as elicitor.

The current study was designed to investigate the impact of the missense Single Nucleotide Polymorphism (SNP), Asn291Ser (c.872A>G: rs12470652), of LHR gene (Luteinizing hormone receptor gene) in peripheral blood samples of Iraqi infertile women diagnosed with premature ovarian failure (POF) and normosmic idiopathic hypogonadotropic hypogonadism(niHH, patients with normal sense of smell). Following the hormonal analysis, fifty women diagnosed with premature ovarian failure and fifty women diagnosed with normosmic idiopathic hypogonadotropic hypogonadism were included as patient groups, while fifty healthy fertile women were enrolled as a control group. The blood samples were obtained from patient and control groups at Kamal Al-Samarrai Hospital in Baghdad, Iraq through March to December 2018. The genotyping of the SNP (rs12470652) was carried out by real-time polymerase chain reaction (real-time PCR) of the purified genomic DNA obtained from All-cell-pellet (ACP) of blood samples. The frequency of the LHR (rs12470652), p.Asn291Ser minor allele G, was found to be 32% healthy fertile women, 30 % of those with POF (p = 0.879), and 28% in the niHH patients (p = 0.644) Therefore, no statistically significant differences were found. This research aims to study the relationship between polymorphism in the region of LHR gene position: 872 A > G, (Asn291Ser), (rs12470652), and both premature ovarian failure and normosmic idiopathic hypogonadotropic hypogonadism. Therefore, the potential influence of (rs12470652) p.Asn291Ser polymorphism on both types of female infertility of an Iraqi population was evaluated. In conclusion, no impact was observed since the conducted study on a sample population of Iraqi women showed that the prevalence of LHR (rs12470652) polymorphisms did not significantly differ in the two infertile patients groups (POF and niHH) as compared to the healthy fertile control group.