Bovine viral diarrhoea virus (BVDV) infection causes severe losses in the cattle population. Viral isolation may have a substantial impact on BVDV prevention and control. This study aimed to isolate and identify the circulating BVDV in Egyptian cows and heifers during 2019. The isolated virus was identified and detected by immune fluorescent assay and immune peroxidase technique followed by RT-PCR that confirmed circulation of noncytopathic BVDV-1 in Egyptian cows and heifers. Further molecular analysis is recommended to determine the molecular epidemiology of BVD isolate for updating vaccine strain as a successful preventive strategy.