Effects of roughage to concentrate ratio on gas production (GP) and nutrient degradability were evaluated in vitro. Rations I: 0R:100C, II: 20R:80C, III: 40R:60C, IV: 60R:40C, V: 80R:20C and VI: 100R:0C were incubated for 24h. Concentrate (0R:100C) produced greater GP/gDM, TDOM and MCP. TDDM was higher for rations I to IV than V and VI. Roughage (100R:0C) had lower PF and higher pH and protozoal count with no VFA effect. Ration III and IV containing 60 and 40% concentrate, respectively exhibited balanced distribution of soluble substrate between microbial biomass production and fermentation waste products and are optimal for sheep nutrition.
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