The present study was aimed to investigate phytoconstituents ethanolic extract of whole plant of Cocculus pendulus using Gas chromatography and mass spectroscopy (GC-MS). GC-MS analysis carried out by using Perkin Elmer clarus 680 model, calrus 680 (EI) and Column Elite-5MS.The GCMS analysis of the ethanolic extract revealed the presence of 16 phytocomponents with valuable biological activities. The major components are Phytol, Oleic acid, Ethyl Iso-allocholate, 14,14A,14B-Octadecahydro-2 , Stigmasterol, Sulfurous acid, Pentadecyl 2-propyl ester 2-methyl-3-(3-methyl-but-2enyl)-2-(4 methyl-pent-3-enyl) Stigmasteryl Tosylate, 9,19-cyclolanost-24-en-3-ol,acetate, (3.beta.)-. 4,22-Stigmastadiene-3-one The identification of different compounds in plant Cocculus pendulus showed that importance in herbal pharmaceutical importance. Finally concluded that investigation of plant revealed up a new perspective in pharmaceutical research and plant can used for various ailments like anticancer flavouring agents, antioxidant antimicrobial, Hepatoprotective, hypolipidemic activities.

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