The present work was developed in the Mayan community of San Sebastian Yaxche, Peto, Yucatan, with the objective of systematizing the traditional agricultural knowledge we found among milperos. Following a qualitative approach, from January 2018 through July 2019, we obtained information through semi-structured interviews with 16 milperos over 40 years of age. The results showed that a large amount of ancestral knowledge in the way their ancestors worked the milpa is still present in older people; therefore, it is urgent that they are transmitted to the younger ones through agricultural work, thus, avoiding their disappearance. This, thanks to the testimonies provided by the people interviewed.