Nitric oxide has been shown to play various physiological and pathological roles in the visual system. We studied here the expression of the neuronal isoform of nitric oxide synthase in the rat superior colliculus and in the dorsal lateral geniculate nucleus after unilateral enucleation, by means of immunohistochemistry, immunoblotting, and real-time PCR. Immunohistochemistry revealed an increase of nitric oxide synthase-positive neurons in specific layers of the superior colliculus and in the dorsal lateral geniculate nucleus between 1 and 30 days post-lesion. Immunoblotting analyses confirmed that the neuronal isoform of nitric oxide synthase is upregulated in the superior colliculus and in the dorsal lateral geniculate nucleus after retinal removal. Diaminofluorescein histochemistry suggested that nitric oxide production was increased in both deafferented retinorecipient areas. Our real-time PCR results indicated that nitric oxide synthase transcript levels in the superior colliculus were not significantly altered after monocular enucleation, although an upregulation of the enzyme transcription was detected into the deafferented dorsal lateral geniculate nucleus. These findings indicated that neuronal nitric oxide synthase may undergo different forms of regulation in the adult deafferented visual system.

Glutamate receptors have been often associated with developmental processes. We used immunohistochemical techniques to evaluate the expression of the AMPA-type glutamate receptor (GluR) subunits in the chick optic tectum (TeO). Chick embryos from the 5th through the 20th embryonic day (E5-E20) and one-day-old (P1) chicks were used. The three types of immunoreactivity evaluated (GluR1, GluR2/3, and GluR4) had different temporal and spatial expression patterns in the several layers of the TeO. The GluR1 subunit first appeared as moderate staining on E7 and then increased on E9. The mature GluR1 pattern included intense staining only in layer 5 of the TeO. The GluR2/3 subunits presented low expression on E5, which became intense on E7. The staining for GluR2/3 changed to very intense on E14 in tectal layer 13. Staining of layer 13 neurons is the most prominent feature of GluR immunoreactivity in the adult TeO. The GluR4 subunit generally presented the lowest expression starting on E7, which was similar to the adult pattern. Some instances of transient expression of GluR subunits were observed in specific cell populations from E9 through E20. These results demonstrate a differential expression of the GluR subunits in the embryonic TeO, adding information about their possible functions in the developmental processes of the visual system. Correspondence