Nitrogen fertilizers applied either in single or split application is the main agronomic inputs to sugarcane production. The objective of this study was to determine the effects of nitrogen fertilizer rates and split application on yield of first ratoon crop of late (CO 421) and early maturing (D 8484) sugarcane varieties. Analysis of variance at p≤0.05 indicated that the mean yields were 117.6 and 124.3 ton cane per hectare for CO* 421 and D* 8484 respectively at harvesting. This study showed that there was no significant difference in the effects of nitrogen fertilizer rate and split application on yield.