Three new 14-membered macrolides, named aspergillides A, B, and C (1, 5, and 7), were isolated from marine-derived fungus Aspergillus ostianus strain 01F313, cultured in a medium composed of bromine-modified artificial seawater. The structures of the new compounds were determined by analyses of 1D and 2D NMR spectra. Their absolute configurations were elucidated by the modified Mosher's method and chemical conversions. The new compounds showed cytotoxic activity against mouse lymphocytic leukemia cells (L1210).

Three novel hybrid polyketide-terpenoid metabolites were isolated from a Penicillium minioluteum strain. Their structures were determined by NMR spectroscopic analyses and X-ray crystallography. The proposed biosynthetic pathway including a unique retro-Claisen migration of methyl carbonate correlates the three compounds with berkeleydione and berkeleytrione.

Three new pentaketides, aspinotriols A ( 1) and B ( 3) and aspinonediol ( 5), were isolated together with two known compounds, aspinonene ( 7) and dihydroaspyrone ( 9), from the marine fungus Aspergillus ostianus strain 01F313, which was collected in Pohnpei and cultured with bromine-modified artificial seawater. The structures of the new compounds were determined by spectroscopic analyses including 1D and 2D NMR. Although 1 and 3 are diastereomers, they show nearly superimposable (1)H and (13)C NMR spectra. The absolute configurations of compounds 1, 3, 5, and 9 were elucidated by the modified Mosher's method.