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SummaryOur GC-MS method for the simultaneous quantitation of sugars and acids as their silyl(oxime) derivatives, from one solution by one injection, has been extended to the reproducible determination of high molecular weight compounds sensitive to decomposition yet requiring a high evaporation temperature (e.g. chlorogenic acid and raffinose) and for the quantitation of the decomposition products of pectin (ie., for the determination of galacturonic acid at low ng levels in the presence of a 10-100 fold excess of glucose eluting just before the acid). The optimized GC procedure has been used for quantitation of the sugar and acid (including chlorogenic acid) composition of potato samples, and for the determination of the increasing amount of the decomposition products of pectin substances in apple pulp after different storage times.

Summary

SummaryA GCNS study was performed in order to determine 1,4-benzoquinone, 1,4-cyclohexanedione, and hydroquinone derivatives one by one and in the presence of each other in different concentrations. Exhaustive investigations, related to the quantitation both of their underivatized compounds and those of their oxime/methoxime and silyl derivatives. Results proved that subsequently to the oximatiodmethoximation of the carbonyl groups -for the sake of a well shaped and accurately evaluable hydroquinone peak -also silylation is unavoidably necessary. Reproducibility data in the microgradnanogram level, hased on quantitation of their total ion currents andor those of their selective fragments, were given in details.

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