This study was conducted to evaluate the immunogenicity and protective efficacy of a DNA vaccine encoding Brucella abortus Cu,Zn superoxide dismutase (SOD). Intramuscular injection of plasmid DNA carrying the SOD gene (pcDNA-SOD) into BALB/c mice elicited both humoral and cellular immune responses. Animals injected with pcDNA-SOD developed SOD-specific antibodies which exhibited a dominance of immunoglobulin G2a (IgG2a) over IgG1. In addition, the DNA vaccine elicited a T-cell-proliferative response and also induced the production of gamma interferon, but not interleukin-10 (IL-10) or IL-4, upon restimulation with either recombinant SOD or crude Brucella protein, suggesting the induction of a typical T-helper-1-dominated immune response in mice. The pcDNA-SOD (but not the control vector) induced a strong, significant level of protection in BALB/c mice against challenge with B. abortus virulent strain 2308; the level of protection was similar to the one induced by B. abortus vaccine strain RB51. Altogether, these data suggest that pcDNA-SOD is a good candidate for use in future studies of vaccination against brucellosis.

Adherent-invasive Escherichia coli (AIEC) strains are genetically variable and virulence factors for AIEC are non-specific. FimH is the most studied pathogenicity-related protein, and there have been few studies on other proteins, such as Serine Protease Autotransporters of Enterobacteriacea (SPATEs). The goal of this study is to characterize E. coli strains isolated from patients with Crohn's disease (CD) in Chile and Spain, and identify genetic differences between strains associated with virulence markers and clonality. We characterized virulence factors and genetic variability by pulse field electrophoresis (PFGE) in 50 E. coli strains isolated from Chilean and Spanish patients with CD, and also determined which of these strains presented an AIEC phenotype. Twenty-six E. coli strains from control patients were also included. PFGE patterns were heterogeneous and we also observed a highly diverse profile of virulence genes among all E. coli strains obtained from patients with CD, including those strains defined as AIEC. Two iron transporter genes chuA, and irp2, were detected in various combinations in 68–84% of CD strains. We found that the most significant individual E. coli genetic marker associated with CD E. coli strains was chuA. In addition, patho-adaptative fimH mutations were absent in some of the highly adherent and invasive strains. The fimH adhesin, the iron transporter irp2, and Class-2 SPATEs did not show a significant association with CD strains. The V27A fimH mutation was detected in the most CD strains. This study highlights the genetic variability of E. coli CD strains from two distinct geographic origins, most of them affiliated with the B2 or D E. coli phylogroups and also reveals that nearly 40% of Chilean and Spanish CD patients are colonized with E.coli with a characteristic AIEC phenotype.

The Internet of Things (IoT) has drawn an enormous attention into the scientific community thanks to unimaginable before applications newly available in everyday life. The technological landscape behind the implied surge of automated interactions among humans and machines has been shaped by plugging into the Internet very low power devices that can perform monitoring and actuation operations through very cheap circuitry. The most challenging IoT scenarios include deployments of low power devices dispersed over wide geographical areas. In such scenarios, satellites will play a key role in bridging the gap towards a pervasive IoT able to easily handle disaster recovery scenarios (earthquakes, tsunamis, and flash floods, etc.), where the presence of a resilient backhauling communications infrastructure is crucial. In these scenarios, Direct-to-Satellite IoT (DtS-IoT) connectivity is preferred as no intermediate ground gateway is required, facilitating and speeding up the deployment of wide coverage IoT infrastructure. In this work, an in-depth yet thorough survey on the state-of-the-art of DtS-IoT is presented. The available physical layer techniques specifically designed for the IoT satellite link are described, and the suitability of both the current Medium Access Control protocol and the upper layer protocols to communicate over space links will be argued. We also discuss the design of the overall satellite LEO constellation and topology to be considered in DtS-IoT networks.

In this paper, we propose an intersection-based connectivity-aware routing protocol (iCAR) for vehicular ad hoc networks (VANETs) to enable infotainment and interactive applications, as well as multi-hop Internet access in urban environments. iCAR is a novel protocol that takes into consideration real-time vehicular traffic information and the experienced packet delivery delay per road, in order to improve the routing performance by dynamically selecting roads with a guaranteed connectivity and a reduced delivery delay. This is achieved by deploying a microscopic view of vehicles location to proactively estimate roads connectivity and the minimum link lifetime per road. Detailed analysis and simulation-based evaluations show that iCAR significantly improves the network performance in terms of packet delivery ratio and end-to-end delay with a negligible cost of communication overhead.978-1-4673-3122-7/13/$31.00 ©2013 IEEE IEEE ICC 2013 -Ad-hoc and Sensor Networking Symposium

Progress in Microgrid (MG) research has evolved the MG concept from classical, purely MG power networks to more advanced power and communications networks. The communications infrastructure helps control and manage the unreliable power outputs that most standard power generation elements of the MG (e.g., wind turbines and photo-voltaic panels) deliver. Although communication technologies do offer certain advantages for sensing and control, they generate other complications due to packet loss and packet latency, among other transmission impairments. In this work, we discuss the impact of communications on MG performance, establishing the requirements of data exchanges and system response in the three levels of a hierarchical control approach: primary, secondary, and tertiary. With a focus on the secondary level-responsible for ensuring the restoration of electrical parameters-we identify standards, networking protocols, and communication technologies relevant for the interoperability of MGs and clusters of MGs, including both modes of operation: isolated and grid-connected. We review theoretical approaches and practical implementations that consider the effects of the communications network on the general performance of the MG. Moreover, we undertake an experimental analysis of the influence of wired and wireless communication networks on MG performance, revealing the importance of designing future smart control solutions more robust to communication degradation, especially if wireless technologies are integrated to provide scalable deployments. Aspects such as resilience, security, and interoperability are also shown to require continuing efforts in research and practical applications.