Sequencing of the left variable region of the L60V Vero cell-adapted strain of African swine fever virus (ASFV) showed the presence of three genes belonging to multigene family 110 (MGF110) and of a fourth unrelated gene. This gene was separated from the MGF110 genes by a region rich in direct repeats. The first MGF110 gene, V1L, with 104 codons, was only moderately related to the other two, W1L and W2L, with 124 and 80 codons, respectively. These two genes were closely related, W2L being a truncated duplication of W1L. Homology matrix analysis of the sequence against itself showed the existence of a repeated block corresponding to the central conserved domain of the three genes, flanked by two other repeated blocks in W1L and W2L. The comparison of the organization of the left variable region of ASFV L60V with that of field isolates and other adapted viruses revealed that adaptation of unrelated viruses resulted in similar large deletions that map, in their right boundaries, exactly at the same positions in the intergenic repeat-rich region.

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Purpose The aim of this study is to identify the main aspects of the decision-making process indicating the use of Augmentative Alternative Communication (AAC), as well as characteristics of the interventions. Methods This is a prospective study, which was submitted to and approved by the Ethics and Research Committee. An electronic questionnaire was created and sent to speech therapist professionals to collect data for this research, with a minimum of 5 years clinical experience in the AAC area. Results We found a predominance in the clinic, of work with young children, especially in cases of ASD and CAS. There is a divergence in the reporting of the addressed technology and the example of the used resource. Most report difficulties in adherence to AAC, mainly due to lack of understanding what AAC is all about and barriers from family members and other professionals. Conclusion There is a lack of research and professional training in the AAC area. Therefore, investing in these areas is necessary to expand the conscious use of AAC and to promote awareness of other professionals and family members.

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