The present investigation entitled “Effect of Growth Regulator (IBA) and Natural Substances the Root and Shoot Growth and Survival Percentage of Pomegranate Cuttings (Punica granatum. L)” was carried out during the year 2021-2022 at the Department of Horticulture, Sam Higginbottom University of Agricultural Technology & Sciences, Prayagraj. The experiment was laid out in Completely Randomized Design (CRD) with nine treatments and three replications. The treatment comprises; T1- Control (water), T2- IBA @1000 ppm, T3- IBA @750 ppm, T4- Vermiwash @100%, T5- Vermiwash @75%, T6- Seaweed extract @100%, T7- Seaweed extract @75%, T8- CPP (Cow Pat Pit) @100%, T9- CPP (Cow Pat Pit) @75%. On the basis of analysis of results, it is found that T4- vermiwash@100% proved best as a potential input for different parameters recorded such as days of first shoot initiation (11), average shoot length (35.78 cm), number of roots per cuttings (38.22), number of leaves per plant (83.89), average root length per cuttings (25.55cm), average leaf area (7.01cm2 ), fresh weight of roots (7.36g), dry weight of roots (3.78g), average shoot girth (3.31cm), average shoot fresh weight (18.89g), average shoot dry weight (9.89g) and survival percentage of pomegranate cuttings in the field after transplanting (93.75%).