Background: COVID-19 cases in Indonesia are still increasing, the increase in cases is also accompanied by an increase in the number of deaths due to COVID-19. Most of the deaths occurred in health workers. Health workers need to have motivation in using self-protection devices to prevent COVID-19 infection.Objectives: Assessing the relationship of knowledge with the motivations of health workers in the prevention of COVID-19.Method: This is a quantitative study with a cross-sectional design. The population of this study is health workers including Doctors, Nurses, Midwives, Health Analysts, and Radiographers. The sampling technique used is quota sampling. Sampling is done to health workers who treat patients with suspicion or have confirmed COVID-19. The statistical test used is the Chi-Square test.Results: Most of the health workers have good knowledge (68.3%) of the prevention of COVID-19. Most health workers have the high motivation (63.4%) for the prevention of COVID-19 in the use of personal protective equipment. There is a link between knowledge and motivation in covid-19 prevention. Conclusion:Health workers who have sufficient knowledge are 8 times more likely to not have the motivation to prevent COVID-19 in using personal protective equipment compared to health workers who have good knowledge