No abstract

No abstract

The present study was objected to investigate isolated flavonoid glycoside from methanolic extract from fruits (Cucumis dipsaceus Ehrenb.) as well as identification. Methanolic extract was already screened for the presence of flavonoid glycoside which was strong antioxidant and hepatoprotective agent. It was first time to isolate these by column chromatography with increasing polarity like Petroleum ether, Chloroform, Ethyl acetate and Methanol solvents of different ratios. Each 20ml elute was collected, analyzed with pre coated Merck F254 TLC plates to compare similar spots and Rf values for same category of contents. The similar elutes were pooled, concentrated dried by vacuum distillation with rotary apparatus. The chloroform ethyl acetate fractions (sticky dark brown mass) were analyzed by pre coated F254 TLC plates and then visualized under visible light, UV 254nm and UV366nm. The developed pre coated TLC plates was further derivatized with 0.5% solution of anisaldehyde - H2SO4 acid and again visualized under visible light and UV366nm.TLC, HPTLC and LCMS methods were used to found and estimate polyphenolic compounds. Spectroscopic methods (IR, 1D and 2D NMR and Mass spectrometry) were used to confirm and elucidate the structure. The concluded structure of flavonoid glycosides was quercetin- 3-rutinoside-7-rhamnoside (M Wt: 756.663g/mol) and plaid with literature data

No abstract

Die Titelverbindung (VII) wird durch Kondensation der Komponenten (V) und (VI) dargestellt und als stabiles Hemiacetal charakterisiert.