The dyes used in the textile industry contribute significantly to pollution of water sources as they are disposed, most of the time, without proper treatment. The objective of this work was to test three strains of two species of the genus Trametes collected in Brazil against the ability to discolor the indigo carmine dye and to detect the activity of the enzymes laccase, lignin peroxidase and manganese peroxidase. The experiment was carried out in Kirk medium under static, non-sterile condition, at ±28 °C for 120 h. Trametes lactinea (URM8350) discolored 81.40% of the indigo carmine dye, T. lactinea (URM8354) 85.09% and T. villosa (URM8022) 96.11%. Laccase was detected in all specimens. Manganese peroxidase was detected in T. villosa (URM8022) and T. lactinea (URM8354), while lignin peroxidase was not detected in any of the isolates under the conditions of the experiment. The discoloration rates observed demonstrate the ability of the strains to discolor carmine indigo and the promising use in the discoloration processes in wastewater from the textile segment.