BACKGROUND For many years Enterococcus species were believed to be harmless commensals of human beings. During the past decade, there has been a worldwide trend in increasing occurrence of Enterococci in the hospitals, a shift in the spectrum of Enterococcal infections and emergence of antimicrobial resistance among such isolates. The steady Pandemic spread of Vancomycin Resistant Enterococci (VRE) along with acquisition of High Level Gentamicin Resistance (HLGR), β-lactamase production and glycopeptides resistance warrants continued surveillance of these versatile pathogens. MATERIALS AND METHODS Therefore, the present study has been carried out on a total no of 2788 clinical specimens (Urine, Pus, Blood and Cerebrospinal fluid) received from different indoor and outpatient departments (