 Abstract: This objective of this paper is primarily focused on RGB color and Gray scale color based key positioning steganography which has been used to overcome the disadvantages of the Least Significant Bit replacement algorithm and helps to embed the audio data in the color images. The given audio data of various sizes is used to embed in the green color channel of the 24 bit color image sequentially by the key based LSB positioning algorithm. Here the audio threshold is another major area where the focus has been laid as increasing the size of the audio data[26] which can be sent through an image without losing the quality of the audio. This method of hiding the audio data through an image helps to authenticate the sender[25] and verifies whether the data has been really sent to the valid user or is used to prevent morphed secret details by the attacker in the middle. The proposed algorithm has been tested against various existing algorithm to study how effectively the algorithm is working, and how effectively it overcomes the drawbacks of the present algorithms. The algorithm is scalable to serve the purpose of authenticating the different demographical region users living all over world and also to identify that the message is reaching only to the valid user[31].