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A BSTR ACTGeneration and negative selection of NK1.1 ؉ ␣͞␤ T cell receptor (TCR) ؉ thymocytes were analyzed using TCR-transgenic (B10.D2 ؋ DO10)F 1 and (C57BL͞6 ؋ DO10)F 1 mice and Rag-1 ؊͞؊ ͞DO10 mice, which had been established by breeding and backcrossing between Rag-1 ؊͞؊ and DO10 mice. Almost all T cells from these mice were shown to bear V␣13͞V␤8.2 that is specific for chicken ovalbumin (cOVA) and restricted to I-A d . A normal proportion of the NK1.1 ؉ V␣13͞V␤8.2 ؉ thymocytes was generated in these mice. However, the actual cell number of both NK1.1 ؉ and NK1.1 ؊ thymocytes in I-A d͞d mice (positive selecting background) was larger than that in I-A b͞d mice (negative selecting background). Markedly low but significant proportions of NK1.1 ؉ V␣13͞V␤8.2 ؉ cells were detected in the spleens from I-A d͞d and I-A b͞d mice. It was shown that the splenic NK1.1 ؉

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