The optical of conjugated polymer poly[(9,9-dioctylfluorenyl-2,7-diyl)-co-(2,5-p-xylene)] also known as [(PFO-co-pX), ADS145UV], dissolved in a few solvents, has been measured. The absorption, emission spectra, and quantum yield have been investigated by using a spectrophotometer and spectrofluorometer, respectively. The properties of Amplified Spontaneous Emission (ASE) of conjugated PFO-co-pX polymer under different conditions such as solvent type, concentration, and pumping energy have been examined by using the tripled frequency of a Nd:YAG laser as a source of pumping. The relation between output energy and pumping energy for the samples with different concentrations in three solvents has been studied. In addition, efficiency and optical gain from the ASE were measured. Additionally, the stability of this polymer as a laser material was investigated. Among the host of conjugated polymer lasers obtained by optical pumping, this conjugated polymer has exhibited only one ASE band under a wide range of concentrations and pump power values. This is most likely due to the steric hindrance by the copolymer segment pX (2,5-p-xylene). This investigation has provided new insights into the excited state dynamics of conjugated polymer materials and has shown that this new conjugated polymer is quite efficient in the violet region.