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As part of the valorization of Aromatic and Medicinal Plants (AMP) in Morocco, we studied the chemical composition and the antibacterial and antifungal activities of Teucrium polium Essential Oil harvested in a region of Pre-Rif, Taza in Morocco (BniKrama). After extraction of the essential oil (EO) by hydro-distillation process, the study of the chemical composition of this EO was carried out by gas chromatography (GPC) and GC/MS (gas chromatography- mass spectrometry), and the evaluation of antibacterial and antifungal activity was carried out against eleven microbial strains by determining the minimum concentration inhibitor. The yield after extraction by hydro-distillation is 0.71%. After (CPG) of the essence of this plant, sixty-eight components have been identified, of which α-Pinene is the major component (21.96%). It is followed by Limonene (18.77%), and β-Pinene (8.46%). The essential oil of T. polium (EOTP) has shown strong antibacterial and antifungal activity. This bioactivity is mainly due to the richness of this essence in terpenes known by their effectiveness against microbial agents. This essential oil is very rich in chemical molecules. Antimicrobial tests show that this EOTP has a broad spectrum on molds, fungi and bacteria.