An eco-friendly protocol for the synthesiso fa zobenzene dyes by oxidative coupling of primary aromatic amines is reported. As efficient biocatalytic systems, CotA laccase and CotA laccase/ABTS (2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)) enablet he oxidationo fv arious substituted anilines, in aqueous medium, ambient atmosphere and under mild reactionconditions of pH and temper-ature. As eries of azobenzene dyes were prepared in good to excellent yields in an one-potreaction. Amechanistic proposal for the formation of the azod erivatives is presented. Our strategy offers an alternative approach for the direct synthesis of azobenzene dyes, avoiding the harsh conditions generally required formost of the traditional synthetic methods.Supporting information and the ORCID identification number(s) for the author(s) of this article can be found under: https://doi.