Aim:The purpose of the study is to determine the thickness of the palatal mucosa by a direct clinical method, and the association of age and gender with the thickness of the palatal mucosa.Materials and Methods:Forty systemically and periodontally healthy Indian subjects participated in the study. The subjects were categorized according to age and gender. The thickness of the palatal mucosa was assessed by bone sounding. Acrylic stents provided 15 consistent locations for the measurement, defined according to the gingival margin and mid-palatal line.Results:The mean thickness of palatal mucosa was 2.48΁0.03 mm, ranging between 2.07 and 3.00 mm, among the participants. The younger age group had significantly thinner mucosa than the older age group. Females had thinner palatal mucosa than males, but not statistically significant.Conclusion:The palatal area from the distal line angle of the canine to the mesial line angle of the palatal root of the first molar provides sufficient donor tissue for grafting procedures. Factors such as body weight and genetic factors need to be further investigated. As the dimension of the soft tissue graft is an important factor influencing the success of the treatment procedure the assessment of the dimension is relevant to clinical periodontics.