Citronella fragrance is one of the many plants in Indonesia. Citronella fragrance is widely used by Indonesian people as a spice dish and has traditionally been used for various treatments. Citronella fragrance contains essential oils that have the potential to have anti-fungal effects and have traditionally been used for the treatment of skin diseases. Isolation of citronella essential oil with the method of vapor distillation. The tested fungi are opportunistic fungi that often infect human skin. In testing the antifungal activity of the citronella essential oil (Cymbopogonnardus (L.) Rendle) against the fungal causing skin infection. The research was done by using agar diffusion method and using the density disc as the diffusion medium against A research of antifungal�s activity of essential oil of citronella (Cymbopogon nardus (L.) Rendle ) to fungus that cause disease in humans has been done. This research used agar diffusion method to the fungus test Tricophyton rubrum, Tricophyton mentagrophytes and Candida albicans. The concentration that used 1%, 0,5%, 0,25% and 0,1%. The results showed that in concentration 1% essential oil of citronella (Cymbopogon nardus ( L.)Rendle) has the highest activity with Candida albicans with an average of inhibition diameter was 19,4mm � 0,15. In 0,1% concentration of essential oil of citronella (Cymbopogon nardus (L.) Rendle) does not have antifungal�s activity to Tricophyton mentagrophytes that without inhibition diameter around the paper discs. Meanwhile, in concentration 0,1%, the essential oil of citronella (Cymbopogon nardus (L.) Rendle) still have antifungal�s activity to Tricophyton rubrum and Candida albicans with an average of inhibition diameter 7,4 mm � 0,35 dan 8,5 mm � 0,15. Keywords: Candida albicans, Sereh wangi (Cymbopogonnardus (L.), Tricophyton rubrum, Tricophyton mentagrophytes, Candida albicans

ABSTRAKTelah dilakukan penelitian tentang efektivitas antiinflamasi ekstrak daun sirsak sebagai komplemen natrium diklofenak pada tikus putih jantan. Pada penelitian ini digunakan 5 kelompok perlakuan yaitu kontrol positif (Tween 80 2%), natrium diklofenak dosis 4,5 mg/kgbb, ekstrak daun sirsak dosis 100 mg/kgbb, kombinasi natrium diklofenak dosis 2,25 mg/kgbb dengan ekstrak daun sirsak dosis 50 mg/kgbb, kombinasi natrium diklofenak dosis 1,125 mg/kgbb dengan ekstrak daun sirsak dosis 25 mg/kgbb. Seluruh kelompok hewan diberikan sediaan uji masing -masing kemudian setelah 1 jam diinduksi radang dengan karagen 1% melalui intraplantar. Selanjutnya dilakukan pengukuran volume telapak kaki sebelum dan sesudah diinduksi radang setiap 30 menit selama 6 jam untuk menghitung persentase radang telapak kaki tersebut. Nilai AUC total dari persentase radang yang diperoleh menjadi parameter untuk menggambarkan aktifitas antiinflamasi pada kelompok perlakuan. Hasil analisis statistik dengan uji T independen menunjukkan bahwa radang yang terbentuk pada kelompok kombinasi natrium diklofenak dan daun sirsak dengan dosis ¼ dosis tunggalnya masing-masing tidak berbeda nyata dengan kelompok yang diberikan dosis tunggal natrium diklofenak sehingga dapat disimpulkan bahwa efek antiinflamasi ekstrak daun sirsak sebagai komplemen pada dosis kombinasi yang lebih kecil tersebut sebanding dengan natrium diklofenak dosis tunggalnya sehingga dapat dikatakan terjadi efek adisi pada kelompok kombinasi tersebut dan ekstrak daun sirsak mampu menurunkan dosis penggunaan natrium diklofenak sebagai antiinflamasi.Kata kunci : Antiinflamasi, komplemen, daun sirsak, natrium diklofenak ABSTRACT

Ekstrak daun sukun menunjukkan adanya efek hepatoprotektor pada penelitian terdahulu sehingga perlu adanya penelitian lanjutan pada beberapa fraksi ekstraknya. Penelitian ini menggunakan 6 kelompok perlakuan yang terdiri dari kontrol normal, kontrol positif, pembanding (sediaan yang mengandung sylmarin 35 mg/kgbb), fraksi etil asetat, fraksi heksan serta fraksi sisa daun sukun dengan dosis masing-masing 47 mg/kgbb. Tiap kelompok perlakuan diberikan sediaan uji selama 7 hari. Selanjutnya kecuali kontrol normal pada hari ke-8 tiap kelompok perlakuan diinduksi hepatotoksik dengan parasetamol dosis 1400 mg/kgbb dan setelah 24 jam dilakukan pengambilan darah melalui arteri carotis untuk diukur aktivitas SGOT

A significant increase in cardiovascular disease prevalence among the Indonesian according to Riskesdas in 2018. Therefore an alternative treatment with minimum side effects to decrease serum total cholesterol is required. Some herbal with anti-hyperlipidemic activity and has a great safety profile can be combined as a mixture to achieve high pharmacology activity with a better taste. Three types of herbal formulas were made from the combination of the juice of garlic, roselle calyx, red ginger, citrus lemon, pineapple, apple cider vinegar, and honey. The variations of the formula were based on the addition of roselle, and pineapple to the composition of garlic, red ginger, citrus lemon, apple cider vinegar, and honey. The doses used in each formula were 2.7 ml/kg BW and 5.4 ml/kg BW. Each formula was given once a day for 10 th day along with high lipid diet-PTU to the animals. Total cholesterol levels were measured digitally through tail vein one day before and after treatment. All herbal juice formula significantly decreased of total cholesterol level of the high lipid diet-PTU rat and it's linear with the dose. The addition of roselle calyx and pineapple each on the herbal juice formula will raise the anti-hyperlipidemic effect. The herbal juice contained roselle showed the highest antihyperlidemic effect at the dose of 5.4 ml/kg BW.