The application of various agricultural technologies such as the using by fertilizers, pesticides, and clearing land will have resulted changes in the ecosystem. Using by insecticides can gived negative impact such as causing resistance, resurgensy and environmental pollution due to residues that can last a long time and are difficult to decompose, because it have compound among other carbon, chlorin, and hidrogen. Piper methysticum (Piperaceae) is one of plant which are used for medicine and social-cultural activitiy by Merauke community, Papua. Meanwhile, Crocidolomia pavonana (Lepidoptera:Crambidae) is one of major pest on the family Brassicaceae plants. The objective of this research was to effectiveness test for simple extract P. methysticum leaves as a botanical insecticide against C. pavonana larvae (Lepidoptera: Crambidae). The insecticide activities were assessed including mortality. Mortality tests using leaf wet method were seven concentrations (5 gr/100 ml, 10 gr/100 ml, 15 gr/100 ml, 20 gr/100 ml, 25 gr/100 ml, 30 gr /100 ml, 35 gr/ 100 ml) plus control. Each treatment was introduced ten C. pavonana instar 2 nd larvaes and replicated five times. The results showed that extract P. methystichum leaves gave 22% mortality at 35 gr/100 ml water.

ABSTRAK Melon (Cucumis melo Linn.) merupakan salah satu tanaman familia Cucurbitaceae yang kemungkinan besar berasal dari Afrika dan tergolong baru beredar di lndonesia. Potongan jaringan darihipokotil mampu diinduksiuntukmembentuktunas secara langsung pada medium padat Murashige dan Skoog, dengan penambahan zat pengatur tumbuh lndoleacetic Acid (lAA) dikombinasikan dengan berbagai konsentrasi Kinetin. Medium yang paling cocok untuk pembentukan tunas adalah medium dengan perbandingan molar Kinetin terhadap IAA 225 kali (Kinetin 5,6 X 1O-5 M; IAR 2,5X 1O-7 Ml. Medium yang paling cocok untuk pertumbuhan tunas adalah medium dengan perbandingan molar Kinetin terhadap IAA 10O kali (Kinetin 2,5 X 10-5 M; IAA 2,5 X 10-7 M). Akar terbentuk pada medium yang mengandung zat pengatur tumbuh lndolebutyric Acid {lBA). ABSTRACTMelon is one of the members of Cucurbitaceae originated from Africa, and is still retributed in lndonesia. By adding combination of lndoleacetic Acid (lAA) with various consentra-