To assess the in vitro biological activity and chemical constituents of Euphorbia perbracteata using different polar solvents, the phytochemical constituents were screened and total phenols, antioxidants, flavonoids content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity and IC 50 values were calculated. Antimicrobial assay was carried out by using disc diffusion method and minimum inhibitory concentration (MIC) was determined against Gram-positive (4), Gram-negative (4) and fungal (1) human pathogenic strains. The results showed that the extracts possessed a broad spectrum of antimicrobial activity with significant MIC values which ranged from 156 to 312 µg/ml. Total phenols (5.163 ± 0.573), antioxidants (2.589 ± 0.115 mg/g Dw) and IC 50 values for DPPH free radical scavenging activity (12.953 µg/ml) was calculated. The observations substantiate the folk claims and the significant biological properties prompted to carry out further studies to isolate the bioactive principles involved in the property which is under progress.