Hypocotyl, cotyledon and cotyledonary node explants of Calendula officinalis L were cultured on Murashige and Skoog (MS) media supplemented with various concentrations of thidiazuron (TDZ), kinetin (KIN), α-naphthaleneacetic acid (NAA) and indole-3-butyric acid (IBA) to induce adventitious shoot regeneration and micropropagation. The highest frequency of adventitious shoot regeneration was achieved from hypocotyl and cotyledon explants on MS media supplemented with 0.75 mg dm -3 TDZ and either 0.25 or 0.50 mg dm -3 IBA. Efficient in vitro clonal propagation was also induced from cotyledonary nodes on a range of media supplemented with 0.75 mg dm -3 TDZ and 0.05 mg dm -3 NAA or 2 mg dm -3 KIN and 1 mg dm -3 NAA. Regenerated shoots were excised and rooted in MS medium supplemented with 1 mg dm -3 NAA. The rooted plantlets were finally transferred to pots.

A procedure has been developed for high frequency adventitious shoot regeneration from lzypocotyls, cotyledon, stem and petiole explants of cicer mil kvetch. All explants isolated from in vitro seedlings were cultured on Murashige and Skoog ( MS) media supplemented with various concentrations of 6-benz_vlaminopurine (BA) and a-naphthaleneacetic acid (NAA) to induce adventitious shoot regeneration. Hypocotyl explants appeared to have better regeneration capacity than stem, cotyledon and petiole explants in most of the media tested. The highest frequency of shoot regeneration was achieved from hypocotyl segments through an initial callus growth on MS medium containing 10 J.IM BA and 0.1 J1M NAA. Regenerated shoots were excised and rooted in half-strength MS medium supplemented with 5.4 J.IM NAA. Rooted plantlets were acclimatized to ambient conditions and later established under greenhouse conditions.

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