An efficient in vitro bulblet production procedure from immature zygotic embryos of endemic and endangered Muscari muscarimi Medik. was described in the current study. Zygotic embryos were first isolated from immature seeds and cultured on different nutrient media compositions supplemented with various combinations of α-naphthalene acetic acid (NAA), picloram, dicamba, 6-benzylaminopurine (BAP), and thidiazuron (TDZ). The best bulblet regeneration (59 bulblets per explant) was achieved in Murashige and Skoog (MS) medium containing 4 mg/L BAP and 0.5 mg/L NAA after 1 year of culture initiation. Regenerated bulblets were then transferred into MS medium without plant growth regulators for rooting. Bulblets produced well-developed root systems and increased their size on this medium after 2 months. All rooted bulblets were successfully transplanted into a potting mixture and acclimatized to ambient conditions.

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In this study, karyotype analysis of the species of Onobrychis sp. was undertaken using the squash method. The results showed that the chromosome number of Onobrychis tournefortii (Willd.) Desv., O. gracilis Besser, O. hypargyrea Boiss. is 2n = 14 and O. argyrea Boiss. subsp. argyrea Boiss., 2n = 16. The karyotype formula of O. tournefortii (Willd.) Desv. is 4m+3sm, of O. hypargyrea Boiss. and O. gracilis Besser 3m+4sm, and of O. argyrea Boiss. subsp. argyrea Boiss. 2m+ 5sm+1 st

This study was conducted to investigate the NaCl impacts on germination and emergence performance of common vetch varieties. Seeds of Gülhan, Selçuk, Kubilay, Emir and Nitra were used as materials. Electrical conductivity (EC) values of NaCl solutions were 5, 10, 15 and 20 dS m-1. Distilled water (0 dS m-1) used as control. In this study germination and emergence test were applied to the varieties. In germination and emergence test mean germination time, mean emergence time, germination percentage, emergence percentage shoot length, root length, shoot fresh and dry weight were measured at the end of the 14th day after sowing. The results showed that germination and emergence percentage and the seedling growth of the varieties were inhibited by NaCl stress and NaCl stress led to increase in germination time and emergence time. Cultivars Gülhan and Emir were the least affected compared to other cultivars. Moreover increased NaCl levels gave rise to increase in Na+ content of cultivars and it was evaluated that the significant correlation (r=-0,525) between Na+ and K+. It was also determined that the correlation between two characters had negative relationship.

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