The study of expression of proteins in organisms on exposure to various environmental challenges gives clues for understanding on how these challenges affects and copes with the biological system. A study was undertaken to understand the proteome profile of Clarias magur, exposed to abiotic stress of water temperature, to find how fishes evolve adaptive strategies towards stress induced by unforeseen vagaries of climate change. Specimens of Clarias magur were exposed to high temperature sub-lethal water stress of 37°C for 60 days and the muscle proteome profiling was analysed through Liquid Chromatography –Mass Spectroscopy for qualitative differential profiling . The study provides an understanding of different proteins expressed as adaptative challenge to the environment. This is the first study to see proteome expression in Clarias magur through Liquid Chromatography –Mass Spectroscopy