Mondia whitei is a popular medicinal and aromatic plant endemic in tropical Africa used in the treatment of various human ailments and for the flavoring of several foods. This paper focuses on determining the best conditions for extraction of bioactive and flavoring extracts from the roots of M. whitei for an efficient use of its virtues. To this end, a characterization of the roots of Mondia whitei was made through the evaluation of its larval toxicity, phytochemical screening, and the search for isovanillin. Then, the surface response method across a centeral composite design was used to determine the extraction conditions. The extraction parameters varied are the extraction temperature (25 to 75 ° C) and the solvent composition (water and methylene chloride: 0 to 100%). The quality of the extracts obtained during the execution of the experimental plan was evaluated through the determination of their vanillin density, anthocyanins, their content of total phenols, and their antioxidant activity. The non-toxicity of M. whitei roots (CL 50> 0.1mg / mL) and the presence of isovalline in the roots were revealed. Phytochemical screening has highlighted the presence of eight phytochemical groups with proven pharmacological activities. Analysis of the variance showed that only the composition of the extraction solvent (water -methylene chloride) had a significant effect (P <0.05) on the quality of the extracts, especially the density in vanillin, in anthocyanins, and the total phenol content. This is with coefficients of determination of 0.753, 0.734 and 0.596 for respective maximum values of 3.00 AU (Absorbance Unit) / g, 1.29 AU / g and 0.426g/100g respectively. In addition, none of the extraction parameters affected the antioxidant activity of the extracts. The determination of the vanillin content of Mondia whitei roots can serve as a quality control marker for Mondia whitei roots. It will be necessary to define other parameters for optimal extraction of the bioactive principles of Mondia whitei roots.