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27Amanitins, highly toxic cyclopeptides isolated from various Amanita species, are the 28 most potent poisons accounting for the hazardous effects on intestinal epithelium cells and 29 hepatocytes, and probably the sole cause of fatal human poisoning. 30The present study was focused on the development, optimization and application of an coefficients of correlation were greater than 0.997 for α-amanitin and 0.993 for β-amanitin. 42Precision was checked at three levels during three consecutive days with intra-day and inter-