Objective: This study was performed to investigate the comparative analysis of ellagic acid content in in vivo (stem, leaf, and root) and in vitro (callus, in vitro developed root and shoot) samples of Oroxylum indicum (L.) Vent. an important medicinal plant. Methods:For in vitro culture, seedling explants were inoculated on MS (Murashige and Skoog's, 1962) medium, supplemented with N6-benzylaminopurine (BAP) and Kn alone and combination with indole acetic acid (IAA). Analytical method high performance liquid chromatography (HPLC) was developed for the quantification of ellagic acid in in vivo and in vitro samples.Result: BAP combination with IAA was best for shoot multiplication, BAP with 2,4-D were used for callus proliferation. HPLC analysis of these extracts revealed that the quantity of ellagic acid present in both the samples, but maximum ellagic acid content was obtained in leaves among all plant parts. Conclusion:Hence, this study showed the in vivo as well as in vitro samples contain ellagic acid. That can be used for its large scale production in future.