Hepatoprotective activity of fennel seeds extract was evaluated on male Wistar rats of 3 mon age. Rats were divided in to 13 groups each containing six rats. Group-I was maintained as normal control. Hepatotoxicity was induced with Carbon tetrachloride 1 ml/kg s.c. subcutaneously in rats of group II. Group-III rats were given standard drug Silymarin@100 mg/kg along with CCl 4 . Rats of Group IV-XIII were given CCL 4 (1 ml/kg, s.c.) Plus methanol and hexane extract of fennel (400 mg/kg/bw, p.o.). Increased levels of ALT, AST, ALP, Bilirubin total and bilirubin direct in blood serum of treated rats were found to reduced by administration of seed extract of different fennel genotypes. Hepatoprotective effect was more evident in total bilirubin and ALP levels where seed extracts of both AF 1 and GF 1 were found to reduce total bilirubin content up to 0.42 and 0.38 respectively from 2.64. Level of ALP was also found to reduce up to 229.15 in hexane extract of genotype RF-101. Similar effect was observed from direct bilirubin content. Hexane seed extract was found more effective than methanol seed extract. A significant genotypic variation was also observed suggested that specific genotypes may be identified to be use for medicinal significance.